Research Interests

Enzymology | Enzyme Discovery | Enzyme Engineering

Marine Microbes | Biochemistry | Interaction

Organic Matter Degradation | Carbohydrates | CAZymes

Metagenomics | Metatranscriptomics | Metaproteomics

 

Academic and Professional Career

Since 11/2023                  Member of GEOMAR’s Commission for Biological Safety /
                                         Biological Safety Delegate (BBS), GEOMAR, Germany

Since 08/2023                  Tenured Staff Scientist at OEB, Marine Ecology Department,
                                         GEOMAR, Germany

Since 03/2022                  Project leader according to §15 (2) GenTSV for OEB’s GMO labs,
                                         Marine Ecology Department, GEOMAR, Germany

08/2020 - 07/2023            Support Scientist at OEB, Marine Ecology Department, GEOMAR,
                                         Germany

08/2020 - 06/2022            Lab Manager at OEB, Marine Ecology Department, GEOMAR,
                                         Germany

01/2020 - 07/2020            Researcher at the Department of Biotechnology and Biomedicine,
                                         Technical University of Denmark, Kongens Lyngby, Denmark

07/2018 - 12/2019            Postdoc at the Department of Biotechnology and Biomedicine,
                                         Technical University of Denmark, Kongens Lyngby, Denmark

04/2016 - 06/2018            Postdoc at the Department of Chemical and Biochemical
                                         Engineering,Technical University of Denmark, Kongens Lyngby,
                                         Denmark

10/2015 - 12/2015            Research Assistant at Enzymical^S AG, Greifswald, Germany

10/2012 - 06/2016            PhD in Biochemistry, University of Greifswald, Germany

05/2011 - 07/2011             IAESTE-Internship at Düag Natural Products, İzmir, Turkey

10/2007 - 08/2012            Diploma degree in Biochemistry, University of Greifswald, Germany

 

List of publications

Articles in peer-review journals

M. Vuillemin, J. Muschiol, Y. Zhang, J. Holck, K. Barrett, J. Preben Morth, A. S. Meyer, B. Zeuner, 2024: Discovery of Lacto-N-biosidases and a Novel N-Acetyllactosaminidase Activity in the CAZy Family GH20: Functional Diversity and Structural Insights, ChemBioChem, e202400710.

C. Bachy, F. Wittmers, J. Muschiol, M. Hamilton, B. Henrissat, A. Z. Worden, 2022: The land-sea connection: insights into the plant lineage from a green algal perspective, Annual Reviews of Plant Biology 73:585-616.

V. T. D. Trang, M. D. Mikkelsen, M. Vuillemin, S. Meier, H. T. T. Cao, J. Muschiol, V. Perna, T. T. Nguyen, V. H. N. Tran, J. Holck, T. T. T. Van, H. H. N. Khanh, A. S. Meyer, 2022: The Endo-α(1,4) Specific Fucoidanase Fhf2 From Formosa haliotis Releases Highly Sulfated Fucoidan Oligosaccharides. Frontiers in Plant Science 13:823668.

M. Vuillemin, J. Holck, M. Matwiejuk, E. S. Moreno Prieto, J. Muschiol, D. Molnar-Gabor, A. S. Meyer, B. Zeuner, 2021: Improvement of the Transglycosylation Efficiency of a Lacto-N-Biosidase from Bifidobacterium bifidum by Protein Engineering, Applied Sciences 11:11493.

M. D. Mikkelsen, H. T. T. Cao, T. Roret, N. Rhein-Knudsen, J. Holck, V. T. T. Tran, T. T. Nguyen, V. H. N. Tran, M. J. Lezyk, J. Muschiol, T. D. Pham, M. Czjzek, A. S. Meyer, 2021: A novel thermostable prokaryotic fucoidan active sulfatase PsFucS1 with an unusual quaternary hexameric structure, Scientific Reports 11:19523.

J. Muschiol, M. Vuillemin, A. S. Meyer, B. Zeuner, 2020: β-N-Acetylhexosaminidases for Carbohydrate Synthesis via Trans-Glycosylation, Catalysts 10:365.

J. Muschiol, L. Munk, K. Li, M. Liu, A. Perzon, S. Meier, P. Ulvskov, A. S. Meyer, 2020: Selective Enzymatic Release and Gel Formation by Cross-Linking of Feruloylated Glucurono-Arabinoxylan from Corn Bran, ACS Sustainable Chemistry & Engineering 8:8164-8174.

B. Zeuner, M. Vuillemin, J. Holck, J. Muschiol, A. S. Meyer, 2020: Improved Transglycosylation by a Xyloglucan-Active α-L-Fucosidase from Fusarium graminearum, Journal of Fungi 6:295.

E. Mnich, N. Bjarnholt, A. Eudes, J. Harholt, C. Holland, B. Jørgensen, F. H. Larsen, M. Liu, R. Manat, A.S. Meyer, J. D. Mikkelsen, M. S. Motawia, J. Muschiol, B. L. Møller, S. R. Møller, A. Perzon, B. L. Petersen, J. L. Ravn, P. Ulvskov, 2020: Phenolic cross-links: building and de-constructing the plant cell wall, Natural Product Reports 37:919-961.

B. Zeuner, D. Teze, J. Muschiol, A. S. Meyer, 2019: Synthesis of Human Milk Oligosaccharides: Protein Engineering Strategies for Improved Enzymatic Transglycosylation, Molecules 24:2033.

J. Muschiol, A. S. Meyer, 2019: A chemo-enzymatic approach for the synthesis of human milk oligosaccharide backbone structures, Zeitschrift für Naturforschung C 74:85–89.

Y. Huang, X. Zheng, B. Pilgaard, J. Holck, J. Muschiol, S. Li, L. Lange, 2019: Identification and characterization of GH11 xylanase and GH43 xylosidase from the chytridiomycetous fungus, Rhizophlyctis rosea, Applied Microbiology and Biotechnology 103:777–791.

R. Mehra, J. Muschiol, A. S. Meyer, K. P. Kepp, 2018: A structural-chemical explanation of fungal laccase activity, Scientific Reports 8:17285.

J. Muschiol, S. B. Jamek, J. Holck, B. Zeuner, P. K. Busk, J. D. Mikkelsen, A. S. Meyer, 2018: Loop Protein Engineering for Improved Transglycosylation Activity of a β-N-Acetylhexosaminidase, ChemBioChem 19:1858–1865.

Z. Zhang, J. Muschiol, Y. Huang, S. B. Sigurdardóttir, N. von Solms, A. E. Daugaard, J. Wei, J. Luo, B.-H. Xu, S. Zhang, M. Pinelo, 2018: Efficient ionic liquid-based platform for multi-enzymatic conversion of carbon dioxide to methanol, Green Chemistry 20:4339–4348.

B. Zeuner, M. Vuillemin, J. Holck, J. Muschiol, A. S. Meyer, 2018: Loop engineering of an α-1,3/4-L-fucosidase for improved synthesis of human milk oligosaccharides, Enzyme and Microbial Technology 115:37–44.

B. Zeuner, J. Muschiol, J. Holck, M. Lezyk, M. R. Gedde, C. Jers, J. D. Mikkelsen, A. S. Meyer, 2018: Substrate specificity and transfucosylation activity of GH29 α-L-fucosidases for enzymatic production of human milk oligosaccharides, New Biotechnology 41:34-45.

S. B. Jamek, C. Nyffenegger, J. Muschiol, J. Holck, A. S. Meyer, J. D. Mikkelsen, 2017: Characterization of two novel bacterial type A exo-chitobiose hydrolases having C-terminal 5/12-type carbohydrate-binding modules, Applied Microbiology and Biotechnology 101(11):4533-4546.

J. Muschiol, C. Peters, N. Oberleitner, M. D. Mihovilovic, U. T. Bornscheuer, F. Rudroff, 2015: Cascade catalysis – strategies and challenges en route to preparative synthetic biology, Chemical Communications 51:5798-5811.

S. Schmidt, C. Scherkus, J. Muschiol, U. Menyes, T. Winkler, W. Hummel, H. Gröger, A. Liese, H.-G. Herz, U. T. Bornscheuer, 2015: An Enzyme Cascade Synthesis of ε-Caprolactone and its Oligomers, Angewandte Chemie International Edition 54(9):2784-2787.

J. Muschiol, H. Mallin, E. Byström, U. T. Bornscheuer, 2013: Efficient Biocatalysis with Immobilized Enzymes or Encapsulated Whole Cell Microorganism by Using the SpinChem Reactor System, ChemCatChem 5(12):3529-3532.

N. Oberleitner, C. Peters, J. Muschiol, M. Kadow, S. Saß, T. Bayer, P. Schaaf, N. Iqbal, F. Rudroff, M. D. Mihovilovic, U. T. Bornscheuer, 2013: An Enzymatic Toolbox for Cascade Reactions: A Showcase for an In Vivo Redox Sequence in Asymmetric Synthesis, ChemCatChem 5(12):3524-3528.

 

Preprints

L. A. Hug, R. Hatzenpichler, C. Moraru, A. Soares, F. Meyer, A. Heyder, The Data Reuse Consortium, A. J. Probst, 2024: A roadmap for fair reuse of public microbiome data, bioRxiv, doi: 10.1101/2024.06.21.599698.

 

Patents

B. Zeuner, J. Muschiol, M. I. Vuillemin, A. M. B. S. Meyer, J. Holck, 2024: Mutated Lacto-N-biosidase, WO 2024/126677 A1.

 

Other publications

J. Muschiol, 2019: Ancestral Sequence Reconstruction – eine neue Methode für die Erzeugung thermostabiler Enzyme? (Journal club contribution), BIOspektrum 25:52.

L. Lange, J. Lindedam, 2017: The Fundamentals Of Bioeconomy: The Biobased Society. (Die Grundlagen der Bioökonomie: Die biobasierte Gesellschaft; German translation in collaboration with IG BAU and J. Muschiol, DTU), published by United Federation of Danish Workers 3F, Order-No: 6075-1.

N. Oberleitner, C. Peters, J. Muschiol, F. Rudroff, M. D. Mihovilovic, U. T. Bornscheuer, 2016: A three Enzyme mediated RedOx Cascade for the production of Normal Carvo-Lactone, in Practical Methods for Biocatalysis and Biotransformations 3 (Eds.: J. Whittall, P. W. Sutton, W. Kroutil), John Wiley & Sons Ltd., pp. 222-226.

H. Mallin, J. Muschiol, E. Byström, U. T. Bornscheuer, 2013: SpinChem: A Novel Reactor Concept For Biocatalysis, BRG Newsletter 12:4-5.