We use tandem (MS/MS) mass spectrometry-based high throughput computational untargeted metabolomics, particularly the Feature-Based Molecular Networking workflow for detailed identification of chemical inventories of marine macro- and microorganisms. Combined with additional bioinformatics tools, these algorithm-based metabolomics can also assist prediction of chemical structures and bioactivity of marine molecules. Comparative metabolomics data (integrated with biological activity) is crucial for prioritization of the most promising samples for chemical work-up, and for monitoring the entire purification process to discover ecologically or biologically active molecules in a targeted manner.

Through exometabolomics/environmental metabolomics approach, we identify, directly, ecologically relevant allelochemicals exuded into seawater to modulate species interactions.

Spatial metabolomics (by DESI Imaging Mass Spectrometry, DESI-IMS), allows us to localize metabolites on biological surfaces and inner tissues, thus delivering information on their site of biosynthesis and potential ecological functions.

Metabolomic analyses are supported by statistical, machine learning and chemometrics tools.

Selected publications:

Tasdemir, D., Scarpato, S., Utermann, C., Jensen, T., Blümel, M., Wenzel-Storjohann, A., Welsch, C. und Echelmeyer, V. A. (2024) Epiphytic and endophytic microbiome of the seagrass Zostera marina: Do they contribute to pathogen reduction in seawater?. Science of the Total Environment, 908 . Art.Nr. 168422. DOI 10.1016/j.scitotenv.2023.168422.

Vitale, G. A., January, G. G., Oppong-Danquah, E. , Della Sala, G., Palma Esposito, F., Tasdemir, D. und de Pascale, D. (2023) A metabologenomics approach to unlock the metabolome of the novel Antarctic deep-sea isolate Lacinutrix shetlandiensis sp. nov. WUR7.  PNAS Nexus, 2 (7). Art.Nr. pgad221. DOI 10.1093/pnasnexus/pgad221.

Fan, B., Grauso, L., Li, F., Scarpato, S., Mangoni, A. und Tasdemir, D. (2022) Application of Feature-Based Molecular Networking for Comparative Metabolomics and Targeted Isolation of Stereoisomers from Algicolous Fungi.  Marine Drugs, 20 (3). Art.Nr. 210. DOI 10.3390/md20030210.

Hassani, M. A., Oppong-Danquah, E., Feurtey, A., Tasdemir, D., Stukenbrock, E. H. und Druzhinina, I. S. (2022) Differential Regulation and Production of Secondary Metabolites among Isolates of the Fungal Wheat Pathogen Zymoseptoria tritici.  Applied and Environmental Microbiology, 88 (6). Art.Nr. e02296-21. DOI 10.1128/aem.02296-21.

Xu, M., Oppong-Danquah, E. , Wang, X., Oddsson, S., Abdelrahman, A., Pedersen, S. V., Szomek, M., Gylfason, A. E., Snorradottir, B. S., Christensen, E. A., Tasdemir, D., Jameson, C. J., Murad, S., Andresson, O. S., Magnusson, K. P., de Boer, H. J., Thorsteinsdottir, M., Omarsdottir, S., Heidmarsson, S. und Olafsdottir, E. S. (2022) Novel methods to characterise spatial distribution and enantiomeric composition of usnic acids in four Icelandic lichens. Phytochemistry, 200. Art.NR. 113210. DOI 10.1016/j.phytochem.2022.113210.

Buedenbender, L. , Kumar, A., Blümel, M. , Kempken, F. und Tasdemir, D.  (2021) Genomics- and Metabolomics-Based Investigation of the Deep-Sea Sediment-Derived Yeast, Rhodotorula mucilaginosa 50-3-19/20B.  Marine Drugs, 19 (1). Art.Nr. 14. DOI 10.3390/md19010014.

Utermann, C., Blümel, M., Busch, K., Buedenbender, L., Lin, Y., Haltli, B. A., Kerr, R. G., Briski, E., Hentschel, U.  und Tasdemir, D.  (2020) Comparative Microbiome and Metabolome Analyses of the Marine Tunicate Ciona intestinalis from Native and Invaded Habitats.  Microorganisms, 8 (12). Art.Nr. 2022. DOI 10.3390/microorganisms8122022.

Papazian, S., Parrot, D., Burýšková, B., Weinberger, F.  und Tasdemir, D.  (2019) Surface chemical defence of the eelgrass Zostera marina against microbial foulers.  Scientific Reports, 9 (1). Art.Nr. 3323 (2019). DOI 10.1038/s41598-019-39212-3.

Parrot, D., Blümel, M., Utermann, C., Chianese, G., Krause, S., Kovalev, A. , Gorb, S. und Tasdemir, D.  (2019) Mapping the Surface Microbiome and Metabolome of Brown Seaweed Fucus vesiculosus by Amplicon Sequencing, Integrated Metabolomics and Imaging Techniques.  Scientific Reports, 9 (1061). DOI 10.1038/s41598-018-37914-8.

Parrot, D., Papazian, S., Foil, D. und Tasdemir, D. (2018) Imaging the Unimaginable: Desorption Electrospray Ionization – Imaging Mass Spectrometry (DESI‑IMS) in Natural Product Research.  Planta Medica, 84 (09/10). pp. 584-593. DOI 10.1055/s-0044-100188.