23.01.2012: FB2-Seminar

 as usual at 13.00 c.t., lecture hall, western shore building

Abstract:

Laser scanning microscopy with 1-photon and 2-photon excitation in combination with reflection and fluorescence techniques allows 3-dimensional investigation of fully hydrated, living bio-films and bio-aggregates.  This imaging approach is able to reveal multi-channel data sets of biological structural features as well as processes and cellular interactions.  The probes available allow assessment of cellular and polymer distribution including the microhabitat.  In this presentation the focus is on traditional laser scanning microscopy in order to demonstrate the versatility of this particular technique with state of the art instrumentation.  Examples are given for: reflection, autofluorescence, lectin-binding analysis, correlative microscopy, 4-dimensional imaging, hydrocarbons, serial scan, mosaic scan, cell compartments, transparent – isosurface, two-photon imaging, rock biofilms, interactions, fluorescent proteins, “high” resolution, deconvolution, process analysis, quantification and modelling.  Then various aspects of running a laser microscopy facility are touched.  Finally a brief outlook is given towards new developments comprising nanoscopy techniques.